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Re: Mice given bat-like forelimbs through gene switch
On Thursday 17 January 2008 01:48:14 Louis Bérubé wrote:
> Can someone please please repost it? I got it truncated.
Here is the abstreact
GENES & DEVELOPMENT 22:141-151, 2008
Regulatory divergence modifies limb length between mammals
Chris J. Cretekos1,2, Ying Wang1, Eric D. Green3, NISC Comparative Sequencing
Program3, James F. Martin4, John J. Rasweiler IV5, and Richard R.
Natural selection acts on variation within populations, resulting in modified
organ morphology, physiology, and ultimately the formation of new species.
Although variation in orthologous proteins can contribute to these
modifications, differences in DNA sequences regulating gene expression may be
a primary source of variation. We replaced a limb-specific transcriptional
enhancer of the mouse Prx1 locus with the orthologous sequence from a bat.
Prx1 expression directed by the bat enhancer results in elevated transcript
levels in developing forelimb bones and forelimbs that are significantly
longer than controls because of endochondral bone formation alterations.
Surprisingly, deletion of the mouse Prx1 limb enhancer results in normal
forelimb length and Prx1 expression, revealing regulatory redundancy. These
findings suggest that mutations accumulating in pre-existing noncoding
regulatory sequences within a population are a source of variation for the
evolution of morphological differences between species and that
cis-regulatory redundancy may facilitate accumulation of such mutations.