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Re: Proteins found in mosasaur bone

I'd like to see what sequencing this does to squamate phylogeny.

On Fri, Apr 29, 2011 at 6:50 PM, bh480@scn.org <bh480@scn.org> wrote:
> From: Ben Creisler
> bh480@scn.org
> New paper in PLoS ONE:
> Lindgren J, Uvdal P, Engdahl A, Lee AH, Alwmark C, et al. (2011).
> Microspectroscopic Evidence of Cretaceous Bone Proteins.
> PLoS ONE 6(4): e19445. doi:10.1371/journal.pone.0019445
> http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0019445
> Low concentrations of the structural protein collagen have recently been
> reported in dinosaur fossils based primarily on mass spectrometric analyses
> of whole bone extracts. However, direct spectroscopic characterization of
> isolated fibrous bone tissues, a crucial test of hypotheses of biomolecular
> preservation over deep time, has not been performed. Here, we demonstrate
> that endogenous proteinaceous molecules are retained in a humerus from a
> Late Cretaceous mosasaur (an extinct giant marine lizard). In situ
> immunofluorescence of demineralized bone extracts shows reactivity to
> antibodies raised against type I collagen, and amino acid analyses of
> soluble proteins extracted from the bone exhibit a composition indicative
> of structural proteins or their breakdown products. These data are
> corroborated by synchrotron radiation-based infrared microspectroscopic
> studies demonstrating that amino acid containing matter is located in bone
> matrix fibrils that express imprints of the characteristic 67 nm
> D-periodicity typical of collagen. Moreover, the fibrils differ
> significantly in spectral signature from those of potential modern
> bacterial contaminants, such as biofilms and collagen-like proteins. Thus,
> the preservation of primary soft tissues and biomolecules is not limited to
> large-sized bones buried in fluvial sandstone environments, but also occurs
> in relatively small-sized skeletal elements deposited in marine sediments.
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