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Brachylophosaurus (hadrosaur) blood vessel mass spectrometry and peptide sequencing

Ben Creisler

A new paper:

Timothy P. Cleland, Elena R. Schroeter, Leonid Zamdborg, Wenxia Zheng,
Ji Eun Lee, John C. Tran, Marshall Bern, Michael B. Duncan, Valerie S.
Lebleu, Dorothy R. Ahl, Paul M. Thomas, Raghu Kalluri, Neil L.
Kelleher, and Mary H. Schweitzer (2015)
Mass Spectrometry and Antibody-Based Characterization of Blood Vessels
from Brachylophosaurus canadensis.
Journal of Proteome Research (advance online publication)
DOI: 10.1021/acs.jproteome.5b00675

Structures similar to blood vessels in location, morphology,
flexibility, and transparency have been recovered after
demineralization of multiple dinosaur cortical bone fragments from
multiple specimens, some of which are as old as 80 Ma. These
structures were hypothesized to be either endogenous to the bone
(i.e., of vascular origin) or the result of biofilm colonizing the
empty osteonal network after degradation of original organic
components. Here, we test the hypothesis that these structures are
endogenous and thus retain proteins in common with extant archosaur
blood vessels that can be detected with high-resolution mass
spectrometry and confirmed by immunofluorescence. Two lines of
evidence support this hypothesis. First, peptide sequencing of
Brachylophosaurus canadensis blood vessel extracts is consistent with
peptides comprising extant archosaurian blood vessels and is not
consistent with a bacterial, cellular slime mold, or fungal origin.
Second, proteins identified by mass spectrometry can be localized to
the tissues using antibodies specific to these proteins, validating
their identity. Data are available via ProteomeXchange with identifier